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G9804-50B Rabbit Anti-Guanylate Cyclase beta-1, Soluble (GCS-beta-1, GC-S-beta-1, GUCY1B1, Guanylate Cyclase 1 Soluble beta 3, GUC1B3, GUCY1B3, Guanylate Cyclase Soluble Subunit beta-3, GCS-beta-3, GC-SB3, GUCB3, GUCSB3, Soluble Guanylate Cyclase Small Subunit)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_000848
Shipping Temp
Blue Ice
Storage Temp
-20°C
EC=4.6.1.2, Guanylate Cyclase Soluble Subunit beta-1

Soluble guanylate cyclase (sGC), a heterodimeric protein consisting of an alpha and a beta subunit, catalyzes the conversion of GTP to the second messenger cGMP and functions as the main receptor for nitric oxide and nitrovasodilator drugs.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-1:500 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide selected from the C-terminal region of human GUCY1B3 (KLH).
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation
Specificity
Recognizes human GUCY1B3.
References
1.Russwurm, M., et al., J. Biol. Chem. 276(48):44647-44652 (2001). 2.Lucas, K.A., et al., Pharmacol. Rev. 52(3):375-414 (2000). 3.Papapetropoulos, A., et al., J. Cell. Physiol. 167(2):213-221 (1996). 4.Giuili, G., et al., Hum. Genet. 91(3):257-260 (1993). 5.Giuili, G., et al., FEBS Lett. 304(1):83-88 (1992).
USBio References
No references available
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