Suitable for use in ELISA and Western Blot. Other applications have not been tested.

Peptide ELISA Titer: 1:128,000 Western Blot: 0.1-0.3ug/ml detects ~150kD band observed in Human Brain lysates (calculated MW of 130kD according to NP_000359.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Miloloza et al Hum Mol Genet. 2000 Jul 22;9(12):1721-7; PMID: 10915759). In transfected HEK293 transiently expressing Human TSC1 a band of approx. 120kD is observed. This band is not observed in the non-transfected HEK293. Optimal dilutions to be determined by the researcher.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Synthetic peptide corresponding to C-QLHIMDYNETHHEH, from the C Terminus of human Hamartin (NP_000359.1).

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

Purified by peptide affinity chromatography.

Recognizes human Hamartin. This antibody is expected to recognise tuberous sclerosis isoform 1 (NP_000359.1) only. Species sequence homology: mouse, rat, canine.