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H4064-05B Goat Anti-HIP-55 (Drebrin-like Protein, Cervical SH3P7, Cervical Mucin-associated Protein, Drebrin-F, HPK1-interacting Protein of 55kD, SH3 Domain-containing Protein 7, DBNL, CMAP, SH3P7, PP5423)

Specifications
References
Clone Type
Polyclonal
Host
Goat
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Ca Hu Mo Rt
Accession #
NP_054782.2, NP_001014436.1, NP_001116428.1
Gene ID
28988, 13169, 83527
Shipping Temp
Blue Ice
Storage Temp
-20°C

DBNL is an F-actin-binding, cytoskeleton adapter protein, and member of the ABP1 family, implicated in the connection of the actin cytoskeleton to endocytic function, through association with the GTPase dynamin. DBNL is degraded in a caspase-dependent manner during apoptosis, and may be involved in the reorganization of the actin cytoskeleton.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:32,000 Western Blot: 1-3ug/ml, observed in Jurkat and 3T3 lysates on ~55kD bands Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to AANLSRNGPALQE-C, from DBNL, at N-terminal (NP_054782.2, NP_001014436.1, NP_001116428.1).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes DBNL. Species Crossreactivity: Human and mouse. Species sequence homology: Canine and rat.
References
1. Ensenat D, Yao Z, Wang XS, Kori R, Zhou G, Lee SC, Tan TH. A novel src homology 3 domain-containing adaptor protein, HIP-55, that interacts with hematopoietic progenitor kinase 1. J Biol Chem. 1999 Nov 26;274(48):33945-50.
USBio References
No references available
Conjugates
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