Suitable for use in Western Blot, Peptide Inhibition, Immunocytochemistry, Chromatin Immunoprecipitation, Beadlyte Histone-Peptide Specificity Assay and Dot Blot. Other applications not tested.

Western Blot: 0.5ug/ml detects dimethylated histone H3 in acid extracted proteins from HeLa cells. No detection of 1ug unmethylated recombinant Histone H3 (H5110-12K) was observed. Peptide Inhibition: 1uM of the immunizing peptide to completely abolishes detection of histone H3 in Western Blot analysis of HeLa acid extracts. Slight signal reduction was observed with peptide containing dimethyl-lysine 9. Signal was not reduced by branched monomethyl H3(Lys27), linear monomethyl H3(Lys27), linear trimethylH3(Lys27), or linear dimethyl H3(Lys4) peptides. Beadlyte Histone-Peptide Specificity Assay: 12.5ng/ml or a range of dilutions detected peptides containing the dimethyl-lysine modification at residue 27. Peptides containing other modifications were not detected. Optimal dilutions to be determined by the researcher.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Synthetic branched peptide containing the sequence ARme2KSA… in which me2K corresponds to dimethyl-lysine at residue 27 of human histone H3 (KLH).

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.

Purified by immunoaffinity chromatography.

Recognizes human dimethylated histone H3 (Lys27), Mr 17kD. At high concentrations, some crossreactivity with dimethyl histone H3 (Lys9) occurs. Broad species crossreactivity expected, based on sequence homology.