Kit Components
H5110-15J1: Histone H4, acetylated (Lys5) 1x25ul H5110-15J2: Histone H4, acetylated (Lys8) 1x20ul H5110-15J3: Histone H4, acetylated (Lys12) 1x25ug H5110-15J4: Histone H4, acetylated (Lys16) 1x20ul
Quality Control Testing Western Blot: Histone H4, acetylated antibodies detected increased levels of acetylated histone H4 in acid extracts of HeLa cells treated with 5mM sodium butyrate. Resolved by electrophoresis, transferred to nitrocellulose and probed with a Histone H4, acetylated antibody, varying dilutions. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.