This antibody has not been tested in methodologies other than Microcytotoxicity Test. Potential applications include Flow Cytometry, Cell Typing, Tissue Staining and Chimerism Studies.

Cytotoxicity: Add 0.9ml of 1% BSA in PBS to 100ul of10X working dilution. Optimal dilutions to be determined by the researcher.

Ascites fluid

Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

HLA Class 2 Antigen-DR52.

Supplied as a lyophilized powder in PBS, 1% BSA. Reconstitute to 100ul sterile dH2O to make a 10X stock solution.

Ascites

Reacts to HLA Class 2 Antigen-DR52. Specificity was determined at a 1:10 dilution by the microcytotoxicity test under standard NIH conditions.