This antibody has not been tested in methodologies other than Microcytotoxicity Test. Potential applications include Flow Cytometry, Cell Typing, Tissue Staining and Chimerism Studies.

Cytotoxicity: Add 0.9ml of 1% BSA in PBS to 100ul of 10X working dilution. Optimal dilutions to be determined by the researcher.

Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction. Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results.

Ascites fluid

Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Supplied as a lyophilized powder from PBS, 1% BSA. Reconstitute with 100ul sterile ddH2O to make a 10X stock solution.

Ascites

Recognizes the HLA Class 2 Antigen-DR7. Specificity was determined at a 1:10 dilution by the microcytotoxicity test under standard NIH conditions.