Suitable for use in Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested.

Immunohistochemistry: Frozen. Increased cytoplasmic staining, relative to nuclear, has been reported using formaldehyde as a fixative compared with acetone/methanol. Optimal dilutions to be determined by the researcher.

Brain

I1904-65W: IgG, F(ab’)2 (HRP) Pab Rb x Mo

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Recombinant protein corresponding to aa2703-2911 of human Huntingtin Protein.

Supplied as a liquid in PBS, 0.09% sodium azide.

Purified by Protein G affinity chromatography.

Recognizes an epitope corresponding to the human HDC region (aa2703-2911) of the Huntingtin protein. Detects a 350kD band on Western Blots but also detects smaller degradation products of Huntingtin. Recognizes both denatured and native Huntingtin in human brain. The combined use of H7960-01, H7960 and H7960-01A demonstrate that huntingtin is enriched in neuronal cells in the brain. Species Crossreactivity: rabbit and mouse.