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H9800-40A Mouse Anti-Hypoxanthine Phosphoribosyltransferase 1 (HPRT1, Hypoxanthine-guanine Phosphoribosyltransferase, HGPRTase, HGPRT, HPRT, Lesch-Nyhan Syndrome)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P00492
Isotype
IgG1,k
Clone Number
12L7
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_000185
Shipping Temp
Blue Ice
Storage Temp
-20°C

HPRT is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. HPRT, which acts as a catalyst in the reaction between guanine and phosphoribosyl pyrophosphate to form GMP, functions primarily to salvage purines from degraded DNA to renewed purine synthesis.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:500-1:5000 Optimal dilutions to be determined by the researcher.
Hybridoma
FO myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant corresponding to aa1-218 from human HPRT expressed in E.coli.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography.|
Specificity
Recognizes human HPRT.
References
1. Sculley DG, et al. (1993). Hum. Genet. 90 (3): 195.
USBio References
No references available
Conjugates
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