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I0402 Rabbit Anti-IARS, NT (Isoleucyl-tRNA Synthetase Cytoplasmic, IARS1, IleRS, ILRS, IRS, FLJ20736, Isoleucine-tRNA Ligase, PRO0785)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
P41252
Isotype
IgG
Grade
Purified
Applications
E IHC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
EC=6.1.1.5

Isoleucine-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in the autoimmune disease polymyositis/dermatomyositis. It catalyzes the chemical reaction: ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

Applications
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Immunohistochemistry: 1:10-1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide selected from the N-term region of human IARS (KLH).
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human IARS.
References
1. Shiba K., Proc. Natl. Acad. Sci. USA 91:7435-7439(1994). 2. Nichols R.C., Gene 155:299-304(1995).
USBio References
No references available
Conjugates
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