Suitable for use in ELISA, Western Blotting, Immunoprecipitation, Immunohistochemistry, Immunoprecipitation. Other applications not tested.
Recommended Dilution
ELISA: 1:40,000 Western Blotting: 1:2000-1:4000 Immunoprecipitation: 1:400 Immunohistochemistry: 1:400 Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Integrin from Geodia cydonium
Form
Supplied as a lyophilized powder in PBS, pH 7.2. Reconstitute in ddH2O.
Wimmer W, Blumbach B, Diehl-Seifert B, Koziol C, Batel R, Steffen R, Müller IM and Müller WEG (1999) Increased expression of integrin and receptor tyrosine kinase genes during autograft fusion in the sponge Geodia cydonium. Cell Adhes. Commun. 7: 111-112
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