Suitable for use in Flow Cytometry. Other applications not tested.

Optimal dilutions to be determined by the researcher.

1. 5x10e5 human Nalm-6 cells were washed incubated 45 minutes on ice with 80ul of I8429-27M1 at 10ug/ml. 2. Cells were washed twice and incubated with IgG (FITC) Goat-anti-Mouse . 3. The cells were washed twice, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.71 log10 fluorescent units when compared to isotype control Mouse IgG1 antibody. Binding was blocked when reagent was pre-incubated with a 10-fold excess of recombinant CD127-muIg (cat# I8429-27N).

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Recombinant correspponding to human CD127.

Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.5mg/ml gentamicin sulfate.

Purified by Protein A affinity chromatography.

Recognizes an extracellular epitope of recombinant and native human CD127 in FACS and ELISA.