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I8451 IPL-52b Insect Medium (Powder)

Specifications
References
Grade
Cell Culture Grade
EU Commodity Code
38210000
Shipping Temp
RT
Storage Temp
RT/4°C

IPL-52b (USDA Insect Pathology Laboratory) for the large scale propagation of cells from the fall armyworm, Spodoptera frugiperda. A modification of Goodwin's orginal IPL formulation, IPL-52b is primarily used for the growth and maintenance of cell lines derived from lepidopterans and the propagation of viruses in these cell lines. See reference for more detail
Goodwin, R.H. Insect cell culture: Improved media and methods for initiating attached cell lines from the lepidoptera. In Vitro 11, 369–378 (1975). https://doi.org/10.1007/BF02616373
Appearance
Off-white, homogenous, free flowing powder
Solubility
Light tan clear, complete
pH
As Reported
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Directions per Liter: Dissolve 30.7g in 800-900ml of ddH2O stirring gently until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Storage and Stability: Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Media Formulation
Inorganic Salts
Ammonium Molybdate•4H2O0.00004
Calcium Chloride•2H2O0.660
Cobalt Chloride•6H2O0.00005
Cupric Chloride•2H2O0.0002
Ferrous Sulfate•7H2O0.00055
Magnesium Sulfate•7H2O1.88
Manganese Chloride•4H2O0.00002
Potassium Chloride2.6
Sodium Bicarbonate 0.350
Sodium Phosphate Monobasic1.16
Zinc Chloride0.00004
Amino Acids
L-Arginine•HCl0.8
L-Asparagine1.3
L-Aspartic Acid1.0
L-Cystine0.1
L-Glutamic Acid1.3
L-Glutamine1.0
Glycine0.4
L-Histidine0.2
Hydroxy-L-Proline0.8
L-Isoleucine0.5
L-Leucine0.4
L-Lysine•HCl0.7
L-Methionine1.0
L-Phenylalanine1.0
L-Proline0.6
DL-Serine0.6
L-Threonine0.2
L-Tryptophan0.1
L-Tyrosine•HCl0.3
L-Valine0.5
Vitamins
Acetyl-b-methylcholine•Cl0.25
p-Aminobenzoic Acid0.00032
D-Biotin0.00016
Vitamin B-120.00024
Folic Acid0.0012
Myo-Inositol0.01
Niacin0.00016
D-Pantothenic Acid•Ca0.00016
Pyridoxine•HCl0.0004
Riboflavin0.00016
Thiamine•HCl0.00016
References
Weiss, S.A., et al., Improved method for the production of insect cell cultures in large volumes. In Vitro 17, 495-502, (1981)
USBio References
No references available
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