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K0005-05L Goat Anti-KLK2 (Kallikrein-2, Glandular Kallikrein-1, hGK-1, Tissue Kallikrein-2)

Specifications
References
Clone Type
Polyclonal
Host
Goat
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu
Accession #
NP_005542.1, NP_001002231.1
Gene ID
3817
Shipping Temp
Blue Ice
Storage Temp
-20°C

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.

Applications
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:16,000 Immunohistochemistry (Paraffin): 2.5-3.8ug/ml Western Blot: 0.1-0.3ug/ml, observed in human prostate lysates on ~22kD bands Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-KHQSLRPDEDSSHD, from human KLK2, at the internal region of the (NP_005542.1, NP_001002231.1).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human KLK2.
References
1. Stephan C, Xu C, Brown DA, Breit SN, Michael A, Nakamura T, Diamandis EP, Meyer H, Cammann H, Jung K. Three new serum markers for prostate cancer detection within a percent free PSA-based artificial neural network. Prostate. 2006 May 1;66(6):651-9.
USBio References
No references available
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