Suitable for Immunoblot (Western or Dot Blot), ELISA,Iimmunoperoxidase Electron Microscopy and Immunohistochemisty. Other applications not tested.

ELISA: 1:1000 to 1:5000 1ug of Lactate Dehydrogenase was used in a standard capture ELISA with ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) A0030 as a substrate for 30 minutes at RT. Optimal dilutions to be determined by the researcher.

Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Lactate Dehydrogenase [Rabbit Muscle]

Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% gentamicin slulfate. Labeled with horseradish peroxidase (HRP).

Purified by ion exchange chromatography.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti goat serum as well as purified and partially purified lactate dehydrogenase (rabbit muscle).