The raw animal product used in the manufacturing of Tryptose Phosphate Broth is derived from casein originating in the USA, New Zealand and/or Australia. The animals used received both ante- and post-mortem inspections under certified veterinary care and were inspected to be free of apparent infectious and contagious diseases. During processing of the raw material, a batch is heated to a minimum of 80°C for 1 hour including a minimum of 5 minutes at 105°C. The product is then dried at a minimum of 140°C.

Appearance: Light yellow to tan, homogeneous free flowing powder

Solubility: Light yellow to yellow, clear, complete

pH: As Reported

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Dissolve 35.6 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. If required, add sodium bicarbonate. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.