Buffered salt formulation used as a base to prepare minimal media for propagation of E. coli and plasmid amplification. A complete media that requires one or more additional salts and carbon source.
| Ammonium Sulfate | 2.0 |
| Potassium Phosphate mono | 13.6 |
| Ferrous Sulfate | 0.0005 |
| Total | 15.6g/L |
| Carbohydrate(glucose, gycerol) | 20g/100ml |
| Add carbohydrate to dH2O. QS to 100ml. Filter sterilize. | |
| MgSO4•7H2O | 24.65g/100ml |
| Add MgSO4•7H2O to dH2O QS to 100ml. Filter sterilize. | |
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