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M2352-40 MAPK Family BioAssay™ Sample Kit

Specifications
References
Brand
BioAssay™
Specificity
Each antibody recognizes only its specific target. The antibodies do not crossreact with one another.
Purity
Purified by Protein A and peptide affinity chromatography.
Kit Type
Assay
Tests
5
Detection Method
Colorimetric/Fluorescent
EU Commodity Code
38220000
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free

P44/42 MAPK (Erk1 and Erk2), SAPK and p38 MAPK function in protein kinase cascades that play a critical role in the regulation of cell growth, and the differentiation and control of cellular responses to cytokines and stress. p44/42 MAPK (Erk1 and Erk2) is activated by growth and neurotrophic factors. Activation occurs through phosphorylation of threonine and tyrosine (202 and 204 of human MAP kinase [Erk1]) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK). SAPK and p38 MAPK are activated by inflammatory cytokines and a wide variety of cellular stresses. Activation of SAPK occurs via phosphorylation at Thr183 and Tyr185 by the dual specificity enzyme SEK/MKK4. Both MKK3 and SEK phosphorylate p38 MAPK on tyrosine and threonine at the sequence T*GY* resulting in p38 activation.

MAPK Family Antibody Sampler Kit contains 20ul of each primary antibody (p44/42 MAP Kinase, SAPK/JNK, p38 MAP Kinase) and 50ul of anti-rabbit IgG secondary antibody labeled with HRP.
Kit Components
M2352-40A: p44/42 MAP Kinase Antibody 1x20ul M2352-40B: SAPK/JNK Antibody 1x20ul M2352-40C: p38 MAP Kinase Antibody 1x20ul M2352-40D: Rabbit ant-Human IgG, HRP, 1x50ul
Applications
Suitable for use in Western Blot and ELISA. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity
Purified by Protein A and peptide affinity chromatography.
Form
Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity
Each antibody recognizes only its specific target. The antibodies do not crossreact with one another.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Lewis, T.S., et al., Adv. Cancer Res. 74: 49-139 (1998). 2. Garrington, T.P., Johnson, G.L., Curr. Opin. Cell. Biol. 11: 211-218 (1999). 3. Schaeffer, H.J., Weber, M.J., Mol. Cell. Biol. 19: 2435-2444 (1999). 4. Whitmarsh, A.J., Davis, R.J., Trends Biochem. Sci. 23: 481-485 (1998). 5. Cobb, M.H., Prog. Biophys. Mol. Biol. 71: 479-500 (1999).
USBio References
No references available
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