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M3852-02 MEM Alpha Modified w/ L-Glutamine, w/o Ribo, Deoxyribonucleosides, Glucose, Adenosine, Cytidine, Pyruvic Acid, Thymidine, Uridine (Powder)

Specifications
References
Grade
Cell Culture Grade
Form
Light orange, homogenous, free flowing powder
EU Commodity Code
38210000
Shipping Temp
RT
Storage Temp
RT/4°C

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed that they had specific nutritional requirements that could not be met by Eagle's Basal Medium (BME). Subsequent studies using these and other cells in culture indicated that additions to BME could be made to aid growth of a wider variety of fastidious cells. MEM, which incorporates these modifications, includes higher concentrations of amino acids so that the medium more closely approximates the protein composition of mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in mono-layers.

Appearance
Light orange, homogenous, free flowing powder
Solubility
Yellow, clear, complete
pH
As Reported
Endotoxin
≤1EU/ml
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Directions per Liter
Dissolve 9.03g in 800-900ml of ddH2O stirring gently until completely solubilized. Adjust pH of the medium to the desired level. If required, add 2.2g/Liter NaHCO3 with stirring. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Media Formulation
Components shown as g/liter
Inorganic Salts
Calcium Chloride•2H2O0.265
Magnesium Sulfate0.09767
Potassium Chloride0.4
Sodium Chloride6.8
Sodium Phosphate Monobasic0.122
Amino Acids
L-Alanine0.025
L-Arginine•HCl0.126
L-Asparagine•H2O0.050
L-Aspartic Acid0.030
L-Cysteine•HCL•H2O0.100
L-Cystine•2HCl0.0313
L-Glutamic Acid0.075
L-Glutamine0.292
Glycine0.050
L-Histidine•HCl•H2O0.042
L-Isoleucine0.052
L-Leucine0.052
L-Lysine•HCl0.0725
L-Methionine0.015
L-Phenylalanine0.032
L-Proline0.040
L-Serine0.025
L-Threonine0.048
L-Tryptophan0.010
L-Tyrosine•Na20.052
L-Valine0.046
Vitamins
L-Ascorbic Acid0.050
D-Biotin0.0001
Choline Chloride0.001
Cyanocobalamin (Vitamin B12)0.00136
Folic Acid0.001
Inositol0.002
Niacinamide0.001
D-Pantothenic Acid•Ca0.001
Pyridoxal•HCl0.001
Riboflavin0.0001
Thiamine•HCl0.001
Other
AdenosineAbsent
CytidineAbsent
2'-DeoxyadenosineAbsent
2'-Deoxycytidine•HClAbsent
2'-Deoxyguanosine•HClAbsent
D-GlucoseAbsent
Guanosine0.01
Pyruvic Acid, SodiumAbsent
Phenol Red, Sodium0.011
Thioctic (Lipoic) Acid0.0002
ThymidineAbsent
UridineAbsent
References
1. Eagle, H., Tissue Culture Association Manual 3:517-520 (1976). 2. Eagle, H. Science 122:501 (1955)
USBio References
No references available
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