Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed that they had specific nutritional requirements that could not be met by Eagle's Basal Medium (BME). Subsequent studies using these and other cells in culture indicated that additions to BME could be made to aid growth of a wider variety of fastidious cells. MEM, which incorporates these modifications, includes higher concentrations of amino acids so that the medium more closely approximates the protein composition of mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in mono-layers.
Components shown as g/liter |
Calcium Chloride•2H2O | 0.265 |
Magnesium Sulfate | 0.09767 |
Potassium Chloride | 0.4 |
Sodium Chloride | 6.8 |
Sodium Phosphate Monobasic | 0.122 |
L-Alanine | 0.025 |
L-Arginine•HCl | 0.126 |
L-Asparagine•H2O | 0.050 |
L-Aspartic Acid | 0.030 |
L-Cysteine•HCL•H2O | 0.100 |
L-Cystine•2HCl | 0.0313 |
L-Glutamic Acid | 0.075 |
L-Glutamine | 0.292 |
Glycine | 0.050 |
L-Histidine•HCl•H2O | 0.042 |
L-Isoleucine | 0.052 |
L-Leucine | 0.052 |
L-Lysine•HCl | 0.0725 |
L-Methionine | 0.015 |
L-Phenylalanine | 0.032 |
L-Proline | 0.040 |
L-Serine | 0.025 |
L-Threonine | 0.048 |
L-Tryptophan | 0.010 |
L-Tyrosine•Na2 | 0.052 |
L-Valine | 0.046 |
L-Ascorbic Acid | 0.050 |
D-Biotin | 0.0001 |
Choline Chloride | 0.001 |
Cyanocobalamin (Vitamin B12) | 0.00136 |
Folic Acid | 0.001 |
Inositol | 0.002 |
Niacinamide | 0.001 |
D-Pantothenic Acid•Ca | 0.001 |
Pyridoxal•HCl | 0.001 |
Riboflavin | 0.0001 |
Thiamine•HCl | 0.001 |
Adenosine | Absent |
Cytidine | Absent |
2'-Deoxyadenosine | Absent |
2'-Deoxycytidine•HCl | Absent |
2'-Deoxyguanosine•HCl | Absent |
D-Glucose | Absent |
Guanosine | 0.01 |
Pyruvic Acid, Sodium | Absent |
Phenol Red, Sodium | 0.011 |
Thioctic (Lipoic) Acid | 0.0002 |
Thymidine | Absent |
Uridine | Absent |
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