Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemstry. Other applications not tested.
Recommended Dilution
Immunohistochemistry (Paraformaldehyde (4%) fixed frozen): 1:10-1:20 Pretreat for 10 min with 20mM glycine in PBS, pH 7.5 and then for 10 min with 6N guanidine hydrochloride in 50mM Tris-HCl, pH 7.5. Western Blot (ECL): 1:10-20 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
pET28a(+) expression vector containing aa408-611 of the p193 cDNA.
Form
Supplied as a liquid, 1% BSA, 0.09% sodium azide.
Purity
Ascites
Specificity
Recognizes an internal epitope (aa491-494, HPGE) of the human minor vault p193 protein. Overexpressed in various human non-P-glycoprotein MDR tumor cell lines, accordingly to an increase in the number of vault particles.
References
1. Schroeijers AB et al., Cancer Res. 60: 1104, 2000; 2.. Kickhoefer VA et al., J. Cell Biol. 146: 917, 1999; 3. Kickhoefer VA et al., J. Bio. Chem. 273: 8971, 1998; 4. Scheper RJ et al., Cancer Res. 53: 1475, 1993; 5. Scheffer GL et al, Nature Med. 1: 578, 1995; 6. Izquierdo MA et al., Cytotechnology 27: 137, 1998.
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