MTHFS

Clone Type

Polyclonal

Host

Goat

Source

Human

Isotype

IgG

Grade

Affinity Purified

Applications

E WB

Crossreactivity

Hu Mo

Accession #

NP_006432.1

Gene ID

10588

Shipping Temp

Blue Ice

Storage Temp

-20°C

Contributes to tetrahydrofolate metabolism. Helps regulate carbon flow through the folate-dependent one-carbon metabolic network that supplies carbon for the biosynthesis of purines, thymidine and amino acids.

Applications

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution

ELISA: 1:32,000 Western Blot: 0.5-1.5ug/ml, observed in human liver lysates on ~25kD bands Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

Synthetic peptide corresponding to KRCLQHQEVKPYT, from human MTHFS, at the internal region of the protein (NP_006432.1).

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

Purity

Purified by immunoaffinity chromatography.

Specificity

Recognizes human MTHFS. Species sequence homology: Mouse.

References

1. Anguera MC, Stover PJ. Methenyltetrahydrofolate synthetase is a high-affinity catecholamine-binding protein. Arch Biochem Biophys. 2006 Nov 15;455(2):175-87. Epub 2006 Oct 4.