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M9505 Murashige and Skoog Minimal Organics Medium w/ Sucrose, Fe EDTA, w/o Ferrous Sulfate (Powder)

Specifications
References
Grade
Plant Grade
EU Commodity Code
38210000
Shipping Temp
RT
Storage Temp
RT

One of the most commonly used plant culture media, Murashige and Skoog Medium has proven effective in the culture of tissue derived from moncotyledons and dicotyledons. This medium, originally formulated to support tobacco callus, has been used to support callus initiation and growth, the growth of cells in suspension culture and the regeneration of shoots and plantlets from explants. Most recently, it has been used for micropropagation of ornamental, vegetable and fruit plants in research and commercial enterprises.

Appearance: White to off-white, homogenous, free flowing powder
Solubility: Clear, colorless, complete
pH: As reported
Directions per Liter
Dissolve 34.5 grams per liter of distilled/deionized (DDI) water. Heat with stirring until completely solubilized. Adjust pH as necessary. If required, add sodium bicarbonate with stirring after solution has cooled. Sterilize with 0.2um membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Storage and Stability
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Media Formulation
Components shown as g/liter
Ammonium Nitrate1.650
Boric Acid0.0062
Calcium Chloride•2H2O0..4398
Cobalt Chloride•6H2O0.000025
Cupric Sulfate0.000016
EDTA Fe•2Na0.0387
Magnesium Sulfate0.18070
Manganese Sulfate•H2O0.01690
Molybdic Acid•Na2•2H2O0.00025
Potassium Iodide0.00083
Potassium Nitrate1.900
Potassium Phosphate Mono0.170
Zinc Sulfate•7H2O0.00860
myo-Inositol0.100
Thiamine•HCl0.00040
Sucrose30.000
References
1. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15: 485 (1962).
USBio References
No references available
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