ATP: AMP phosphotransferase (EC 2.7.4.3) from yeast
Reaction Equation
ATP + AMP = ADP + ADP
Specific Activity
> 200U/mg protein (at 25°C and pH7.5)
Unit Definition of Activity
One unit is defined as the amount of enzyme that will convert 1.0umol ATP and 1.0umol AMP to 2umol ADP per min at 25°C, pH 7.5. Note: 1 KU = 1000 units.
Contaminants
ATPase ≤ 0.01% Phosphoglycerate kinase ≤ 0.1%
Storage and Stability
Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Form
Supplied in 50% Glycerol solution
Specificity
Recognizes > 200u/mg protein at 25°C and pH7.5.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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