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N0008-37 NANS, aa1-359, Recombinant, Human (Sialic Acid Synthase, N-acetylneuraminate Synthase, N-acetylneuraminate-9-phosphate Synthase, N-acetylneuraminic Acid Phosphate Synthase, N-acetylneuraminic Acid Synthase, SAS) CAS:

Specifications
References
Grade
Highly Purified
Accession Number
NP_061819
Molecular Weight
42.4
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C

NANS, also known as SAS, is a 359aa protein that contains one AFP (antifreeze proteins)-like domain and functions in the biosynthesis of sialic acids. Expressed ubiquitously, NANS enzymatically catalyzes the H2O-dependent formation of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), both of which are sialic acids. NANS uses N-acetylmannosamine 6-phosphate as a substrate for Neu5Ac synthesis and mannose 6-phosphate as a substrate for KDN synthesis.

Source
Recombinant corresponding to aa1-359 of human NANS protein, fused to His-tag at N-terminus, expressed in E.coli.
AA Sequence
MGSSHHHHHH SSGLVPRGSH MPLELELCPG RWVGGQHPCF IIAEIGQNHQ GDLDVAKRMI RMAKECGADC AKFQKSELEF KFNRKALERP YTSKHSWGKT YGEHKRHLEF SHDQYRELQR YAEEVGIFFT ASGMDEMAVE FLHELNVPFF KVGSGDTNNF PYLEKTAKKG RPMVISSGMQ SMDTMKQVYQ IVKPLNPNFC FLQCTSAYPL QPEDVNLRVI SEYQKLFPDI PIGYSGHETG IAISVAAVAL GAKVLERHIT LDKTWKGSDH SASLEPGELA ELVRSVRLVE RALGSPTKQL LPCEMACNEK LGKSVVAKVK IPEGTILTMD MLTVKVGEPK GYPPEDIFNL VGKKVLVTVE EDDTIMEELV DNHGKKIKS
Molecular Weight: 42.4kD (379aa) confirmed by MALDI-TOF
Enzyme Activity
Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Purity
~95% (SDS-PAGE).
Concentration
~0.5mg/ml
Form
Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.1M sodium chloride, 1mM DTT, 10% glycerol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Milstein O., et al. (2008) J Biol Chem. 283(49):34414-22. 2. Aldape MJ., et al. (2007) J Infect Dis. 195(12):1838-45
USBio References
No references available
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