NANOS1

Clone Type

Polyclonal

Host

Goat

Source

Human

Isotype

IgG

Grade

Affinity Purified

Applications

E WB

Crossreactivity

Accession #

NP_955631.1

Gene ID

340719

Shipping Temp

Blue Ice

Storage Temp

-20°C

May act as a translational repressor which regulates translation of specific mRNAs by forming a complex with PUM2 that associates with the 3'-UTR of mRNA targets. Capable of interfering with the proadhesive and anti-invasive functions of E-cadherin. Up-regulates the production of MMP14 to promote tumor cell invasion.

Applications

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution

ELISA: 1:64,000 Western Blot: 1-3ug/ml, observed in human testis lysates on ~35-37kD bands Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

Synthetic peptide corresponding to C-SARDGPPGKKLR, from human NANOS1, at C-terminal (NP_955631.1).

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

Purity

Purified by immunoaffinity chromatography.

Specificity

Recognizes human NANOS1.

References

1. Jaruzelska J, Kotecki M, Kusz K, Spik A, Firpo M, Reijo Pera RA. Conservation of a Pumilio-Nanos complex from Drosophila germ plasm to human germ cells. Dev Genes Evol. 2003 Apr;213(3):120-6. Epub 2003 Feb 11.