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N0574-80B Goat Anti-NEDD1 (Protein NEDD1, Neural Precursor Cell Expressed Developmentally Down-regulated Protein 1, NEDD-1)

Specifications
References
Clone Type
Polyclonal
Host
Goat
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_690869.1
Gene ID
121441
Shipping Temp
Blue Ice
Storage Temp
-20°C

Required for mitosis progression. Promotes the nucleation of microtubules from the spindle.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:32,000 Western Blot: 1-3ug/ml, observed in lysates of cell line HeLa on ~75kD bands Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-NQTRNSEKFEKPEN, from human NEDD1, at the internal region of the protein (NP_690869.1).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human NEDD1.
References
1. Haren L, Remy MH, Bazin I, Callebaut I, Wright M, Merdes A. NEDD1-dependent recruitment of the gamma-tubulin ring complex to the centrosome is necessary for centriole duplication and spindle assembly. J Cell Biol. 2006 Feb 13;172(4):505-15. Epub 2006 Feb 6.
USBio References
No references available
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