NELL2

Clone Type

Monoclonal

Host

Mouse

Source

Human

Isotype

IgG2b,k

Clone Number

12L1

Grade

Affinity Purified

Applications

E WB

Crossreactivity

Hu Mo

Accession #

NP_006150

Shipping Temp

Blue Ice

Storage Temp

-20°C

Protein kinase C-binding protein, NELL2 is expressed abundantly in neural tissues and may play important roles in the development of neural tissues. Also, NELL protein contains six epidermal growth factor (EGF)-like repeat domains that participate in calcium binding.

Applications

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution

Western Blot: 1:500 Optimal dilutions to be determined by the researcher.

Hybridoma

FO myeloma cells with spleen cells from Balb/c mice.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

Recombinant corresponding to aa30-258 from human NELL2 expressed in E.coli.

Form

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

Purity

Purified by Protein G affinity chromatography.

Specificity

Recognizes human NELL2. Species Crossreactivity: mouse.

References

1. Kuroda S, et al. (1999) Biochem. Biophys. Res. Commun. 265 (1): 79. 2. Luce MJ, Burrows PD. (1999) Gene 231 (1-2): 121-6. 3. Watanabe TK, et al. (1997). Genomics 38 (3): 273-6.