Murine netrin-1 precursor is a 67.768 Kd protein (604aa) expressed by subpopulations of cells within the developing CNS, as well as in various non- neural tissues, including the gut, liver, heart, and prostate. In the embryonic CNS, netrin-1 is expressed by cells of the floor plate, and acts to attract axons of commissural neurons from the dorsal horn gray matter. Netrin-1 is also believed to play a role in the growth of motor axons in the PNS.
Applications
Suitable for use in Immunohistochemistry and Immunocytochemistry. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry: 1:1000-1:2000 using 2% paraformaldehyde tissues Immunocytochemistry: 1:1000-1:2000 using 2% paraformaldehyde cells Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies
I1906 IgY, Chicken Pab Rb x Ch I1906-10 IgY, Chicken (HRP) Rb x Ch I1906-12 IgY, Chicken (FITC) Rb x Ch I1906-14 IgY, Chicken (AP) Rb x Ch
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to Netrin-1 (KLH). Species sequence homology: human (O_095631, NCBI) and mouse (O_09118, NCBI)
Form
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes Netrin-1. Species crossreactivity: mouse. Species sequence homology: human.
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