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N2008-05B Rabbit Anti-Neomycin Phosphotransferase II (NPT II)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Swiss Prot
P00552
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Shipping Temp
Blue Ice
Storage Temp
4°C Do not freeze

Neomycin phosphotransferase II confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin®, G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Western Blot analysis of transfected cell lysates with anti-NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids.

Applications
Suitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended Dilutions
Western Blot: Detects NPT II in RIPA lysates from Cos-1 cells transfected with pUSE, harboring the neomycin resistance gene Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Full length Neomycin Phosphotransferase II (NPT II).
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes bacterial NPT II, Mr ~30kD.
References
1. Qian, Y., et al., Biochem. J. 415: 123-134 (2008). General References: 1. Fuchs, R.L., et al., Biotechnology 11: 1537-1542 (1993). 2. Fuchs, M. and Gonsalves, D., Biotechnology 13: 1466-1473 (1995). 3. Horn, D. and Cross, G.A.M., Cell 83: 555-561 (1995). 4. Hu, H., et al., Neuron 16: 735-743 (1996).
USBio References
US Biological Application References: 1. Qian, Y., et al., Biochem. J. 415: 123-134 (2008).
Conjugates
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