N-myc Downstream Regulated Gene 1 Protein, phosphorylated

Clone Type

Monoclonal

Host

Rabbit

Source

Mouse

Swiss Prot

Q62433

Isotype

IgG

Clone Number

11C1051(D98G11)

Grade

Purified

Applications

FC IC IF IHC WB

Crossreactivity

Hu Mk Mo Rt

Gene ID

10397

Shipping Temp

Blue Ice

Storage Temp

-20°C

NDRG1 (N-myc downstream-regulated gene 1), also termed Cap43, Drg1, RTP/rit42, and Proxy-1, is a member of the NDRG1 family comprised of four members (NDRG1-4) that function in growth, differentiation and cell survival. NDRG1 is ubiquitously expressed and highly responsive to a variety of stress signals including DNA damage, hypoxia and elevated levels of nickel and calcium.

Applications

Suitable for use in Immunofluorescence/Immunocytochemistry, Flow Cytometry, Western Blot and Immunohistochemistry . Other applications not tested.

Recommended Dilutions

Immunofluorescence (IC): 1:800 Flow Cytometry: 1:200 Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunohistochemistry (Paraffin): 1:500. Requires HIER using sodium citrate buffer pH 6.0 prior to staining. Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Thr346 of mouse NDRG1.

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, and 50% glycerol.

Purity

Purified.

Specificity

Recognizes mouse NDRG1, phosphorylated at Thr346. |Crossreactivity: May cross-reacts with other conserved phosporylation sites on NDRG1 at positions Thr356 and Thr366.|Species Crossreactivity: human, monkey and rat.

References

1. Shimono, A. et al. (1999) Mech Dev 83, 39-52. 2. Zhou, D. et al. (1998) Cancer Res 58, 2182-9. 3. van Belzen, N. et al. (1997) Lab Invest 77, 85-92 4. Kurdistani, S.K. et al. (1998) Cancer Res 58, 4439-44. 5. Park, H. et al. (2000) Biochem Biophys Res Commun 276, 321-8. 6. Li, J. and Kretzner, L. (2003) Mol Cell Biochem 250, 91-105. 7. Stein, S. et al. (2004) J Biol Chem 279, 48930-40. 8. Maruyama, Y. et al. (2006) Cancer Res 66, 6233-42. 9. Nishio, S. et al. (2008) Cancer Lett 264, 36-43. 10. Kalaydjieva, L. et al. (2000) Am J Hum Genet 67, 47-58. 11. Okuda, T. et al. (2004) Mol Cell Biol 24, 3949-56. 12. Taketomi, Y. et al. (2007) J Immunol 178, 7042-53. 13. Murray, J.T. et al. (2004) Biochem J 384, 477-88.

USBio References

No references available

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N2915-57 Rabbit Anti-N-myc Downstream Regulated Gene 1 Protein, phosphorylated (Thr346) (NDRG1, Ndr1, Ndrl, Protein NDRG1, Protein Ndr1, CAP43, CMT4D, DRG1, GC4, HMSNL, NMSL, PROXY1, RIT42, RTP, TARG1, Tdd5)

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