Nourseothricin CAS 96736-11-7

CAS Number

96736-11-7

Grade

Highly Purified

Molecular Formula

(D) C31H58N12O10 · (F) C19H34N8O8 · (E) C25H46N10O9 · (C) C37H70N14O11 · H2SO4

Molecular Weight

-758.86502526306988704

EU Commodity Code

38220090

Shipping Temp

Blue Ice

Storage Temp

-20°C

NTC; Nurseotricin; Nourseotricin; clonNAT; Streptothricin Sulfate

A group of antibiotic substances produced by Streptomyces variants. They are composed of an amino sugar and different polypeptide chains and may have broad spectrum antimicrobial and some antiviral properties. Streptothricin antibiotic Nourseothricin (NTC) is used for a broad spectrum of viruses, bacteria and other unicellular or complex organisms. Preferred selection antibiotic for genetically modified Gram-positive and Gram-negative bacteria, yeast, filamentous fungi, protozoa, plants and many more. Selection of recombinant strains is based on inactivation of NTC by monoacetylation of b-amino group of the b-lysine by Nouresothricin N-acetyltransferase the product of the sat1 or nat1 genes. Nourseothricin is a mixture of four streptothricin compounds: D and F (~90%) as well as C and E (~10%)

Synonyms

NTC; Nurseotricin; Nourseotricin; clonNAT; Streptothricin Sulfate

CAS No

96736-11-7

Molecular Formula

(D) C31H58N12O10 · (F) C19H34N8O8 · (E) C25H46N10O9 · (C) C37H70N14O11 · H2SO4

Molecular Weight

Streptothricin D (758.86), Streptothricin F (502.52), Streptothricin E (630.69), Streptothricin C (887.04)

Form

Supplied as a non-sterile powder. Reconstitute with ddH2O to 100mg/ml and sterile filter. Maximal solubility is 1g/ml.

Purity

Components D&F (HPLC): ~90%; Components C&E (HPLC): ~10%

Biological Activity

~110% standard Nr. 82

Ash

~2%

Elementary Analysis (Typical)

C: 32.3% H: 5.7% N: 14.0% S: 6.7%

Applications

Suitable for use in selection of recombinant Leishmania strains.

Recommended Dilutions

Added to growth medium to a final concentration of 100ug/ml. Optimal dilutions to be determined by the researcher.

Reconstitution

Reconstitute with ddH2O to 100mg/ml and sterile filter. Maximal solubility is 1g/ml.

Selection

For selection of recombinant Leishmania strains Nourseothricin NTC is added to the growth medium to a final concentration of 100ug/ml.

PubChem Chemical ID

Streptothricin D: 202550, Streptothricin F: 197034

Table 1

* MIC: Minimal inhibitory concentration ** IC50: Concentration inhibiting growth by 50%

Group Species MIC* Selection conc. ug/ml ug/ml Gram-negative bacteria Escherichia coli 2-12 50 Francisella tularensis - 5 Pseudomonas aeruginosa 50 100 Gram-positive bacteria Bacillus subtilis 5 50 Enterococcus faecium 8-256 500 Staphylococcus aureus -12 50 Streptomycetes Streptomyces lividans 6 100 Yeast Candida albicans 200 250-450 Cryptococcus neoformans - 100 Hansenula polymorpha - 100 Pichia pastoris - 100 Saccharomyces cerevisiae 25 100 Filamentous fungi Acremonium chrysogenum - 25 Sordaria macrospora - 50 Ustilago maydis - 75 Protozoa Leishmania tarentolae - 100 Phytomonas serpens - 100 Plasmodium falciparum 75* - Toxoplasma gondii - 500 Plants Arabidopsis thaliana 20 200 Oryza sativa 20 200

Storage and Stability

Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Toxicity and Hazards

All products should be handled by qualified personnel only, trained in laboratory procedures.

References

1. Cox, et al., Infect. Immunity 71: 173 (2003). 2. Gold, et al., Gene 142: 225 (1994). 3. Goldstein, et al., Yeast 15: 1541 (1999). 4. Hamano, et al., J. Biol. Chem. 281: 16,842 (2006). 5. Hentges, et al., Yeast 22: 1013 (2005). 6. Jacobs, et al., Nature Protocols 4: 58 (2009). 7. Ji, et al., J. Antibiot. 60: 739 (2007). 8. Joshi, et al., Gene 156: 145 (1995). 9. Kloti, et al., Selectable marker in plants. United States Patent 6696621 (2004). 10. Kojic, et al., Can. J. Microbiology 46: 333 (2000). 11. Kück, et al., Fungal Genetics Newsletter 53: 9 (2006). 12. Lukeš, et al., Mol. Biochem. Parasitol. 148: 125 (2006). 13. Maier, et al., Appl. Environment. Microbiol. 72: 1878 (2006). 14. McDade, et al., Med. Mycol. 39: 151 (2001). 15. Mamoun, et al., PNAS 96: 8716 (1999). 16. Mandal, et al., Ind. J. Exptl. Biol. 47: 475 (2009). 17. Nagotu, et al., Traffic 9: 1471 (2008). 18. Roemer, et al., Molec. Microbiol. 50: 167 (2003). 19. Shen, et al., Infect. Immun. 73: 1239 (2005). 20. Van, et al., J. Parasitol. 92: 668 (2006). 21. Werner, et al., Antimicrob. Agents Chemother. 45: 3267 (2001).

USBio References

No references available

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N5375-74 Nourseothricin (NTC, Nurseotricin, Nourseotricin, clonNAT, Streptothricin Sulfate) CAS: 96736-11-7

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NTC; Nurseotricin; Nourseotricin; clonNAT; Streptothricin Sulfate

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