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N7100-43A Rabbit Anti-NUP54, ID (Nucleoporin p54, 54kD Nucleoporin)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
Q7Z3B4
Isotype
IgG
Grade
Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_059122
Shipping Temp
Blue Ice
Storage Temp
-20°C

The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. NUP54 is a member of the phe-gly (FG) repeat-containing nucleoporin subset.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-1:500 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide selected from the center region of human NUP54 (KLH).
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human NUP54.
References
1.Stoffler, D., et al., Curr. Opin. Cell Biol. 11(3):391-401 (1999). 2.Bodoor, K., et al., Biochem. Cell Biol. 77(4):321-329 (1999). 3.Popov, S., et al., J. Biol. Chem. 273(21):13347-13352 (1998). 4.Hu, T., et al., J. Cell Biol. 134(3):589-601 (1996). 5.Finlay, D.R., et al., J. Cell Biol. 114(1):169-183 (1991).
USBio References
No references available
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