Nutrient Broth

Grade

Molecular Biology Grade

EU Commodity Code

38210000

Shipping Temp

Storage Temp

RT/4°C

Beef Extract, Peptone

Nutrient Broth is a basic media composed of a simple peptone and a beef extract. Peptone contributes organic nitrogen in the form of amino acids and long-chained fatty acids. Beef Extract provides additional vitamins, carbohydrates, salts and other organic nitrogen compounds. If needed other enrichments may be added during the preparation of N8530 or to prepared media.

Appearance

Light tan, homogeneous, free flowing powder

Solubility

Yellow to gold, clear, complete

7.0±0.2

Directions per Liter

Dissolve 8 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).

Storage and Stability

Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Media Formulation

Components shown as g/liter

Beef Extract	3
Peptone	5

Total:

8g/liter

References

1. Ausubel, F.M., et al., Current Protocols in Molecular Biology, John Wiley (1992). 2. Maniatis, T., et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press (1989).