Ovalbumin (abbreviated OVA) is the main protein found in egg white, making up 60-65% of the total protein. Ovalbumin displays sequence and three-dimensional homology to the serpin superfamily. The function of ovalbumin is unknown, although it is presumed to be a storage protein or carrier protein.
Applications
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution
ELISA: 1:1000-1:5000 Western Blot: 1:500-1:2500 Optimal dilutions to be determined by the researcher.
Biotin/Protein Ratio
10-20 moles Biotin/mole of IgG
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Ovalbumin (Hen Egg White).
Form
Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Reconstitute with 100ul ddH2O. Labeled with Biotin.
Purity
Purified by Ion Exchange chromatography.
Specificity
Recognizes chicken ovalbumin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-rabbit serum as well as purified and partially purified Ovalbumin (Hen Egg White.
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UniProt
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