Carbohydrate Fermentation Study: 1% final carbohydrate concentration with phenol red (0.018g/L) indicator and Durham tube. After incubation a yellow broth with or without gas bubbles indicates a positive fermentation reaction. Indole Test: Place an indole test strip 10mm above a 24-48 hr. broth culture. Reincubate for 5-30 minutes. A violet red color on the test strip indicates a positive reaction.

Store unopened medium at 2-30°C. Store opened medium container in a low humidity environment at 2-30°C. Store liquid medium at 4°C. The powder medium should be discarded if it is not free flowing or if the color has changed from the original light beige color.

Appearance: Light beige, homogeneous, free flowing powder Solubility: Gold to amber, clear to turbid to clear.. pH: 7.2 ± 0.2

Microbiological Analysis:Escherichia coli (ATCC 25922): Growth Salmonella typhimurium (ATCC 14028): Growth Staphylococcus aureus (ATCC 25923): Growth

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Directions per Liter Dissolve 15.0 grams per liter of distilled/deionized water, heating with stirring until completely solubilized, adjusting pH as necessary. If required, add sodium bicarbonate with stirring when solution has cooled. Sterilize with 0.2µ membrane filter. Aliquot into sterile containers.

Storage Store at 0-5ºC (store unopened media at 2-30°C). Opened bottles should be capped tightly and kept in a dark, low humidity environment.