Phosphatase Assay Kit, Alkaline/Acid RRA-pS-VA, BioAssay™

Brand

BioAssay™

Kit Type

Assay

Tests

100

Detection Method

Colorimetric/Fluorescent

Detection Range

0.5U of calf intestine alkaline phosphatase released 66.9pmol phosphate per minute

Sample Matrix

Cell extracts

EU Commodity Code

38220000

Shipping Temp

Blue Ice

Storage Temp

4°C

This Phosphatase Assay Kit, Alkaline/Acid RRA-pS-VA, BioAssay™ is suitable for detection and quantitation of acid and alkaline phosphatase activity from cell extracts.

Kit Capacity

100 assays per kit.

Kit Components

Malachite Green Solution A Malachite Green Additive Phosphate Standard Serine Phosphopeptide (RRApSVA) pNPP (p-Nitrophenyl Phosphate) NiCl2, 40mM pNPP Ser/Thr Assay Buffer 96-well microtiter plate

Quality Control

The components of this kit have matched to optimize sensitivity and range of detection. Phosphate released is determined by comparing absorbance to standard curve of step 2. Using this kit, 0.5U of calf intestine alkaline phosphatase released 66.9pmol phosphate per minute using the phosphopeptide (RRApSVA).

Note

Do not perform the Malachite Green Assay using phosphate buffers or with biological samples that contain high levels of free phosphate. Remove free phosphate from samples by desalting column or dialysis.

Storage and Stability

Store kit at 4°C. Stable for 1 year after receipt.

Kit Components

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

References

1. Harder, K.W., et al., Biochem. J. 298: 395-401 (1994). 2. Donella-Deana, A., et al., Biochim. Biophys. Acta 1094: 130-133 (1991). 3. Donella-Deana, A., et al., Biochim. Biophys. Acta 1051: 199-202 (1990). 4. Ek man, P. & Jager, O., Annal. Biochem. 214: 138-141 (1993).

USBio References

No references available

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P4071-60 Phosphatase Assay Kit, Alkaline/Acid RRA-pS-VA, BioAssay™

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Tests

Detection Method

Detection Range

Sample Matrix

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References

USBio References

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