Lot Specific. Sample Lot Value: 4,176 pmol/min/ug.

One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′-AMP per min at 37°C. Assay Conditions: 10mM Tris-HCl, pH 7.4, 10mM MgCl2, 1mM MnCl2, 200uM cAMP, and 0.2ng/ul PDE4A1A at 37°C for 20 min. Quantified by 5′-nucleotidase cleaving the 5′-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Optimal dilutions to be determined by the researcher.

Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

Sf9 insect cells

>70% by SDS-PAGE

As reported

Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM NaCl, 2.2mM KCl, 16mM glutathione, 3mM DTT and 20% glycerol

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.