Suitable for use in Immunoprecipitation/Western Blot. Other applications have not been tested.
Recommended Dilutions
Immunoprecipitation/Western Blot: 5ug immunoprecipitated PLC b-1 from a bovine brain membrane preparation. The immunoprecipitation procedure was followed by an Western Blot using 5ug/ml of P4074-40 and a chemiluminescence detection system. Optimal dilutions to be determined by the researcher.
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Mixed monoclonal antibodies raised against purified bovine PLCβ-1
Form
Supplied as a liquid in 100mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes bovine PLC b-1 at 150kD. Also recognizes the 100kD and 41kD fragments of PLC b-1. Species Crossreactivity: rat, mouse and human.
References
1. Suh, P.G., et al., J. Biol. Chem. 263: 14497–14504, 1988. 2. Rhee, S.G., et al., Proc. Natl. Acad. Sci. USA 88: 5453, 1991. 3. Meisenhelder, J., Cell 57: 1109–1122, 1989. 4. Ryu, S.H., J. Biol. Chem. 262: 12511–12518, 1987. 5. Liu, et al, J Biol. Chem. 271: 355, 1996. 6. Kind, P.C., et al., J. Neurosci. 17: 1471-1480, 1987
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