Basalt salt mixture used in the preparation of Pichia pastoris fermentation medium. Manufactured from ACS Grade components and pre-blended to complete homogeneity. Less expensive than buying the individual chemicals!
Appearance
White to light tan, homogeneous, free flowing powder
Solubility
Colorless, clear, complete
Directions per Liter
Dissolve 34.03 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Components shown as g/liter |
Calcium Sulfate Dihydrate | 0.93 |
Magnesium Sulfate | 14.9 |
Potassium Sulfate | 18.2 |