Suitable for use in ELISA, Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000-1:5000. A band of 95-110kD has been detected in brush border membrane (2). ELISA: 1:10,000-50,000. Control peptide can be used to coat ELISA plates at 1ug/ml. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
A 19 AA synthetic peptide within the 4th putative cytoplasmic domain of Human PiT-2/GLVR-2 (1) was synthesized, conjugated to KLH.
Form
Supplied as a liquid in PBS, 0.05% sodium azide, 40% glycerol.
Purity
Serum
Specificity
Recognizes human PiT 2. Species sequence homology: rat, hamster (100%), mouse and feline (94%). No significant sequence homology is observed with PiT-1 or NaPi-Type I, -2, or IV transporters.
References
(1). Van Zeijl M et al (1994) PNAS 91, 1168-1172; Miller DG et al (1994) PNAS 91, 78-82; Bai L et al (2000) Am. J. Physiol. 279, C1135-C1143; (2) Boyer CJC et al (1998) BBA 1368, 73-83; Fernandes I et al (1999) Am. J. Physiol. 277, F543-F551; (3) Murer H et al (2000) Physiolo. Rev. 80, 1373 (review); Werner A et al (2001) Am. J. Physiol. Integ. Comp. Physiol. 280, R301 (review).
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