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P4258-23A Mouse Anti-Platelet Derived Growth Factor Receptor, Type B (PDGFRb) (APC)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P09619
Conjugate
APC
Isotype
IgG1
Clone Number
7H36
Grade
Affinity Purified
Applications
FC
Crossreactivity
Hu Mk
Gene ID
5159
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

PDGF receptor beta is a member of the class III receptor tyrosine kinase family that also includes M-CSF receptor, SCF receptor and Flt-3. Binding of PDGF-BB induces receptor homodimerization or heterodimerization with PDGF receptor alpha.

Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 10ul labels 1x10e6 cells Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunogen
Human skin fibroblast cell membrane extracts
Form
Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein G affinity chromatography from ascites.
Specificity
Recognizes the extracellular domain of human Platelet Derived Growth Factor Receptor, Type B. Does not recognize PDGF receptor alpha. Species Crossreactivity: baboon and monkey. Does not react with mouse or rat
References
1. Hart, et al. (1987) J. Biol. Chem. 262:10780. 2. Gronwald, et al. (1988) Proc. Natl. Acad. Sci. USA 85:3435. 3. Seifert, et al. (1989) J. Biol. Chem. 264:8771. 4. Heldin, C.H. and L. Claesson-Welsh (1994) in Guidebook to Cytokines and Their Receptors, Nicola, N.A. ed. Oxford University Press, New York, p. 202.
USBio References
No references available
Conjugates
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