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P4379 Tween 20 (Polysorbate 20, Polyoxyethylenesorbitan Monolaurate) CAS: 9005-64-5

Specifications
References
CAS Number
9005-64-5
Grade
Molecular Biology Grade
Molecular Weight
1227.54
EU Commodity Code
38220090
Shipping Temp
RT
Storage Temp
RT
Polysorbate 20; Polyethylene glycol sorbitan monolaurate

Tween-20 is a polysorbate surfactant whose stability and relative non-toxicity allow it to be used as a detergent and emulsifier in a number of domestic, scientific, and pharmacological applications. It is a polyoxyethylene derivative of sorbitan monolaurate, and is distinguished from the other members in the Tween range by the length of the polyoxyethylene chain. The commercial product contains a range of chemical species.

Biological Applications
Tween 20 has a broad range of applications. For example, it is used: to stabilize purified protein derivative (PPD) solution, used in skin testing for tuberculosis exposure, as a blocking agent in immunologic assays, such as Western blots and ELISA and as a solubilizing agent of membrane proteins for lysing mammalian cells, at a concentration of 0.05 to 0.5%.
CAS No
9005-64-5
Synonyms
Polysorbate 20; Polyethylene glycol sorbitan monolaurate
Appearance
Yellow to amber viscous liquid.
Hydroxyl Number
96-108
Water (KF)
≤3%
Specific Gravity (25°C)
1.080-1.120
Acid Value
≤2.2mg KOH/g
Saponification Value
≤50mg KOH/g
Residual Solvents (1, 4-Dioxane)
≤0.001%
RNase, DNase (1.5% and 3%)
None Detected
Storage and Stability
Store at RT.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Toxicity and Hazards
 All products should be handled by qualified personnel only, trained in laboratory procedures.
References
1. Ayorinde FO, Gelain SV, Johnson JH Jr, Wan LW. (2000). "Analysis of some commercial polysorbate formulations using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry". Rapid Communications in Mass Spectrometry 14 (22): 2116-2124. PMID 11114018. Retrieved on 7 August 2006.. 2. Joint FAO/WHO Expert Committee on Food Additives (1974). Toxicological evaluation of some food additives including anticaking agents, antimicrobials, antioxidants, emulsifiers and thickening agents. WHO Food Additives Series No. 5. World Health Organization. Retrieved on 7 August 2006. 3. Stephens AD, et al. Chromatin and lamin A determine two different mechanical response regimes of the cell nucleus. Mol Biol Cell. 2017 Jan 5. pii: mbc.E16-09-0653. doi: 10.1091/mbc.E16-09-0653. [Epub ahead of print]
USBio References
1. Stephens AD, et al. Chromatin and lamin A determine two different mechanical response regimes of the cell nucleus. Mol Biol Cell. 2017 Jan 5. pii: mbc.E16-09-0653. doi: 10.1091/mbc.E16-09-0653. [Epub ahead of print]
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