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P5250-01K Goat Anti-POU2F1 (POU Domain, Class 2, Transcription Factor 1, NF-A1, Octamer-binding Protein 1, Oct-1, Octamer-binding Transcription Factor 1, OTF-1, OCT1, OTF1)

Specifications
Clone Type
Polyclonal
Host
Goat
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Bo Ca Hu Mo Po Rt
Accession #
NP_002688.2
Gene ID
5451
Shipping Temp
Blue Ice
Storage Temp
-20°C

OCT1 is a transcription factor that binds the octamer motif 5'-ATTTGCAT-3' in promoters of various genes.OCT1 contains one homeobox DNA binding domain and is part of the POU transcription factor family due to the presence of one POU domain, a highly charged motif that selectively binds the octamer motif.OCT1 is also known as POU domain class 2 transcription factor, octamer-binding transcription factor, oct-1, OTF-1, NF-A1, and POU2F1.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:4000 Western Blot: 0.5-2ug/ml, nuclear lysates of cell line HeLa observed on ~100kD bands Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-NVQSKSNEESGDSQQ, from human POU2F1, at the internal region, near N-terminal (NP_002688.2).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human POU2F1. Species sequence homology: Bovine, canine, mouse, porcine and rat.
Conjugates
Pricing
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