POU2F3

Clone Type

Polyclonal

Host

Goat

Source

Human

Isotype

IgG

Grade

Affinity Purified

Applications

E WB

Crossreactivity

Bo Ca Hu Rt

Accession #

NP_055167.2

Gene ID

25833

Shipping Temp

Blue Ice

Storage Temp

-20°C

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Regulated the expression of a number of genes such as SPRR2A or placental lactogen.

Applications

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution

ELISA: 1:32,000 Western Blot: 1-3ug/ml, lysates of cell lines HEK293 and HepG2 observed on ~50kD bands Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

Synthetic peptide corresponding to C-EPHLEASQHLPVPKH, from human POU2F3, at the internal region of the protein (NP_055167.2).

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

Purity

Purified by immunoaffinity chromatography.

Specificity

Recognizes human POU2F3. Species sequence homology: Bovine, canine and rat.

References

1. Kukimoto I, Mori S, Sato H, Takeuchi T, Kanda T, Transcription factor human Skn-1a enhances replication of human papillomavirus DNA through the direct binding to two sites near the viral replication origin. The FEBS journal 2008 Jun 275(12):3123-35.

USBio References

No references available

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P5250-06A Goat Anti-POU2F3 (POU Domain, Class 2, Transcription Factor 3, Octamer-binding Protein 11, Oct-11, Octamer-binding Transcription Factor 11, OTF-11, Transcription Factor PLA-1, Transcription Factor Skn-1, OTF11, PLA1)

Clone Type

Host

Source

Isotype

Grade

Applications

Crossreactivity

Accession #

Gene ID

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References

USBio References

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