May play a role in determining or maintaining the identities of a small subset of visual system neurons.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:8000 Western Blot: 0.2-0.6ug/ml, human and mouse heart lysates observed on ~40kD bands Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-EAAYREKNSKPE, from POU4F3, at the internal region of the protein (NP_002691.1).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human POU4F3. Species Crossreactivity: Mouse. Species sequence homology: Canine and rat.