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P9054-08X Mouse Anti-Prostate Specific Antigen (PSA, Kallikrein-3, Kallikrein III, Seminin, Semenogelase, gamma-Seminoprotein, P-30 antigen, KLK3) (HRP)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
HRP
Isotype
IgG1,k
Clone Number
12K277
Grade
Affinity Purified
Applications
E
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

PSA is a chymotrypsin-like serine protease (kallikrein family) produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia. Anti-PSA is useful in the positive identification of metastatic deposits of prostatic origin.

Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Matched Pair
Capture: P9054-08A
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human PSA
Form
Supplied as a liquid in 0.01M PBS, pH 7.0, 50% glycerol. Labeled with horseradish peroxidase (HRP).
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human PSA in normal prostate, benign prostate hyperplasia and prostate carcinoma.
References
1. Sadagopan Krishnan et al. Attomolar Detection of a Cancer Biomarker Protein in Serum by Surface Plasmon Resonance Using Superparamagnetic Particle Labels* Angew. Chem. Int. Ed. Engl. 2011 February 1; 50(5): 1175–1178.|2. Vigneshwaran Mani et al. Ultrasensitive Immunosensor for Cancer Biomarker Proteins using Gold Nanoparticle Film Electrodes and Multienzyme-Particle Amplification. ACS Nano. Mar 24, 2009; 3(3): 585–594.|3. Mani V et al. Highly efficient binding of paramagnetic beads bioconjugated with 100,000 or more antibodies to protein-coated surfaces. Anal Chem. 2012 Dec 4;84(23):10485-91.|4. Bhaskara V. Chikkaveeraih et al. Single-Wall Carbon Nanotube Forest Arrays For Immunoelectrochemical Measurement of 4 Protein Biomarkers for Prostate Cancer. Anal. Chem. Nov 1, 2009; 81(21): 9129-9134.|5. Bhaskara V. Chikkaveeraiah et al. Microfluidic Electrochemical Immunoarray for Ultrasensitive Detection of Two Cancer Biomarker Proteins in Serum. Biosens Bioelectron. Jul 15, 2011; 26(11): 4477-4483.|6. Jeong-Mi Moon et al. A nanowire-based label-free immunosensor: Direct incorporation of a PSA antibody in electropolymerized polypyrrole. Biosensors and Bioelectronics. Volume 57, 15 July 2014, Pages 157–161.|7. Gary C. Jensen et al. Characterization of Multienzyme-Antibody-Carbon Nanotube Bioconjugates for Immunosensors. J Nanosci Nanotechnol. Jan 2009; 9(1): 249–255.|8. Chikkaveeraiah BV, Mani V, Patel V, Gutkind JS, Rusling JF.Microfluidic electrochemical immunoarray for ultrasensitive detection of two cancer biomarker proteins in serum. Biosens Bioelectron 2011; 11(26):4477-83.
USBio References
No references available
Conjugates
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