Proteasome 26S, P52 Subunit

Clone Type

Monoclonal

Host

Mouse

Source

Human

Isotype

IgG1

Clone Number

5F284

Grade

Supernatant

Applications

Crossreactivity

Hu Rt Xe

Shipping Temp

Blue Ice

Storage Temp

-20°C

The p52 subunit (Mr 52,000) is the major antigen of the 19S cap complex, which is a subcomplex of the 26S proteasome, implicated in ATP- and ubiquitin-dependent protein degradation.

Applications

Suitable for use in Western Blot. Not recommended for Immunofluorescence and Immunoprecipitation. Other applications not tested.

Recommended Dilution

Western Blot: 1:5-1:10 when using the ECL method. Dilution buffer: 0.15M PBS with 0.1% BSA and 0.1% sodium azide. Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

26S proteasomes purified from Xenopus laevis ovary

Form

Supplied as a liquid, 0.09% sodium azide

Purity

Hybridoma culture supernatant

Specificity

Recognizes Proteasome 26S, P52 Subunit. Species Crossreactivity: Xenopus laevis; XLKE-A6 (X. laevis). Cultured Cell Lines: PLC, A-431, MCF-7, CaCo (human); RVF (rat); Ptk2 (kangaroo rat). Does not react with 3T3 (mouse).

References

Peters JM, Franke WW, and JA Kleinschmidt: distinct 19S and 20S subcomplexes of the 26S proteasome and their distribution in the nucleus and the cytoplasm. J Biol Chem 269:7709-7718 (1994)|JM Peters: Proteasomes: protein degradation machines of the cell. TIBS 19, September 1994

USBio References

No references available

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P9078-65 Mouse Anti-Proteasome 26S, P52 Subunit

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