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P9100-15 Purification Media, Divinyl Sulfone (DVS) Beads, BioSeparation™ CAS:

Specifications
Brand
BioSeparation™
Grade
Molecular Biology Grade
EU Commodity Code
38220090
Shipping Temp
RT
Storage Temp
4°C Do not freeze

Divinyl� Sulfone� (DVS)� Activated� Beads �are �rigid �Sepharose� beads� loaded� with� high� density� of� reactive� groups.� The� matrix� is� suitable� for� binding �amino �or �hydroxyl� groups, �present� on� biomolecules �such �as �proteins �and� carbohydrates. �The �DVS� activated� beads� are� stable� for �extended� period �of �time� when �stored �refrigerated� and �light-protected.� Coupling� reactions� to� biomolecules� may �be �done� between �pH �values �of �8-10.���

Matrix
Sepharose�� CL-4B�
Activation �Method:� Divinyl�Sulfone
Binding �Capacity
2-4�mg BSA/�ml
Bead �Size:� 40-165um�
Bead �Structure:� Highly �cross-linked �spherical �agarose, �4%�
Max� Back� Pressure:� 0.3MPa ,�3�bar�
Max Flow �Rate
15�ml/min/cm2�
Buffers� required �for� protein conjugation (using �10ml� DVS Activated Beads)
Recommended �Flow Rate:� 8-12�ml/min/�cm2� � �(Scale� up� or �down �all �values �when �conjugating �more� or �less �than� 10�ml �of� beads�) DVS� Activated �Beads:�10ml Double� distilled �water: 100ml Neutral �conjugation �buffer �(50ml):� Phosphate �Buffered �Saline� (PBS) �pH �6-8�� Conjugation �buffer� (50ml):� 0.1N �Sodium� bicarbonate� buffer �(pH-8-10).�
Storage and Stability
Store gel refrigerated in any desired buffer added with 0.1% (w/v) sodium azide until use. Use within 6 months after receipt.
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