Superior Prime Broth™ is made without animal-derived raw materials and is APF Certified. The media are complex, rich formulations which supply a nitrogen source, vitamins, and moderate glucose levels. The media are buffered at pH 7.2±0.2 to prevent acidification of the medium during metabolism of the glucose.

For expression of recombinant proteins, induction should be done at cell densities twice that of LB Broth. Cell yields are typically about 3 to 4 times that of LB Broth in shake flask cultures depending on the characteristics of the strain.  Plasmid copy numbers are the highest among the pEX™ family of media. This medium is an excellent choice for large-scale plasmid preparations. Biomass yields will depend on the process employed and the strain.

Our experiences with the expression of recombinant proteins have shown that the level of expression is very much dependent on the medium used. Some proteins are expressed well in one medium, but are not expressed in the same host strain in another medium or protein may be expressed in one medium while another protein is not expressed in the same medium.

This demonstrates why for each recombinant protein a medium screen becomes a crucial part of the optimization process. To facilitate the researchers’ efforts to develop the best systems for their proteins, we have developed a Media Optimization Kit.™. The kit is composed of six media formulations (including four that are unique and proprietary) which we have found over the years to be the most reliable for the increased production of recombinant proteins in Escherichia coli. The kit is used to quickly and easily identify the most suitable medium for the expression of a particular recombinant protein. Included are suggested approaches for improving the production of recombinant proteins, thus passing along the knowledge our staff scientists have acquired while working to produce a wide variety of proteins.

Amber, clear, complete

7.3±0.2

No detectable microbial growth.

Store at 4°C. Do Not Freeze. Stable for 6 months after receipt.

P9102-40: Media Optimization Kit™ P9102-40A: Turbo Broth™ P9102-40B: Superior Broth™ P9102-40C: Power Broth™ P9102-40D: Hyper Broth™ P9102-40E: M9Y (Glucose) Broth™ P9102-40F: Glucose-Nutrient Mix P9102-40G: LB Broth (Miller)