USBio Logo

P9102-40J Protein Expression Media, LB Booster™ (Powder) pEX™

Specifications
References
Brand
pEX™
Grade
Molecular Biology Grade
EU Commodity Code
38210000
Shipping Temp
RT
Storage Temp
RT/4°C

LB Booster™ is a medium additive which increases the level of production of recombinant proteins.Particularly suitable for improving the productivity of LB Broth. LB Booster™ can increase biomass levels by a factor of ± 5 in any medium formulation.In addition, the supplement provides essential nutrients selected for their ability to increase the relative level of recombinant protein accumulation. LB Booster™ is supplied as a powder and as a ready-to-use 20x concentrate. LB Booster™ is not a glucose supplement.

pEX™ Protein Expression Media P9102-40 Media Optimization Kit™ P9102-40A Turbo Broth™ P9102-40B Superior Broth™ P9102-40C Power Broth™ P9102-40D Hyper Broth™ P9102-40E M9Y (Glucose) Broth™ P9102-40F Glucose-Nutrient Mix P9102-40G LB Broth (Miller)
Not sure which media expression system to choose? United States Biological has developed a unique research tool for protein expression media selection: Media Optimization Kit™
Our experiences with the expression of recombinant proteins have shown that the level of expression is very much dependent on the medium used. Some proteins are expressed well in one medium, but are not expressed in the same host strain in another medium or protein may be expressed in one medium while another protein is not expressed in the same medium.
This demonstrates why for each recombinant protein a medium screen becomes a crucial part of the optimization process. To facilitate the researchers’ efforts to develop the best systems for their proteins, we have developed a Media Optimization Kit.™. The kit is composed of six media formulations (including four that are unique and proprietary) which we have found over the years to be the most reliable for the increased production of recombinant proteins in Escherichia coli. The kit is used to quickly and easily identify the most suitable medium for the expression of a particular recombinant protein. Included are suggested approaches for improving the production of recombinant proteins, thus passing along the knowledge our staff scientists have acquired while working to produce a wide variety of proteins.
Appearance
White, homogenous, free-flowing, fine powder.
Solubility
Clear, transparent, complete
Recommended Dilution
Add 5ml LB Booster™ to 95ml LB Broth.
Directions
1. Dissolve 2.4g of LB Booster™ in 10ml dH2O. Stir with gentle heating to dissolve completely. 2. Filter sterilize using 0.2um membrane. 3. Store liquid at 4°C
References
1. Luria, S.E., Burrous, J.W., Hybridization between Escherichia coli and Shigella. J. Bacteriol. 74: 461-476 (1955). 2. Lennox, E.S., Transduction of linked genetic characters of the host by bacteriophage P1. Virology 1: 190-206 (1955). 3. Luria, S.E., Adams, J.N., Ting, R.C., Transduction of lactose-utilizing ability among strain of E. coli and S. dysenteriae and the properties of the transducing phage particles. Virology 12:348-390 (1960). 4. Miller, J.H., Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York (1972). 5. Sambrook, J., Fritsch, E.F., Maniatis, T., Molecular cloning: a laboratory manual, 2nd edition. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York (1989).
USBio References
No references available
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
  • Contact Us

    Visit our technical library or contact our support staff to answer your questions.

    Telephone:
    1-800-520-3011

    Library | Contact

    Distributors

    For customers outside of the United States, please use one of our many distributors.

    View Distributors

    Payment Methods

    We accept the following payment methods as well as pay-by-invoice.

    MasterCard Visa PayPal
    © 2023-2024 United States Biological - All Rights Reserved